This section tackles progressive numerical problems based on the rate of photosynthesis, focusing on oxygen evolution rates, the compensation point, and identifying limiting factors.
Photosynthetic Equation:6CO2+12H2OChlorophyll⟶SunlightC6H12O6+6O2+6H2O(Note the specific conditions required for the reaction, represented above and below the arrow).
Rate of Photosynthesis: Calculated by measuring the oxygen (O2) evolution over time (e.g., bubbles per minute).
Rate=Time TakenNumber of O2 Bubbles
Net vs. Gross Photosynthesis: Net Photosynthesis = Gross Photosynthesis - Respiration.
Question:
During a laboratory experiment using the Hydrilla funnel setup, a student observes that 120 bubbles of oxygen are released over a period of 15 minutes under bright sunlight. Calculate the average rate of photosynthesis per minute.
Step-by-Step Solution:
Identify the Given Data:
Total Oxygen Bubbles = 120
Total Time = 15 minutes
Apply the Rate Formula:Rate=Total TimeTotal Bubbles
Calculation:Rate=15120=8 bubbles/minute
Answer: The average rate of photosynthesis is 8 bubbles per minute.
Question:
A potted plant is kept in a closed glass bell jar. Sensors monitor the total O2 and CO2 levels inside the jar. At a specific low light intensity (at dawn), the sensors record that the net change in CO2 concentration inside the jar is precisely zero.
A student concludes: "Photosynthesis has completely stopped because there is no CO2 uptake."
Is the student correct? Justify your answer numerically using the concept of rates.
Common Pitfall:
Students often confuse Net Photosynthesis with Gross Photosynthesis. They assume zero net change means zero metabolic activity.
Step-by-Step Solution:
Identify the biological processes: A plant simultaneously undergoes two processes:
Respiration: Consumes O2, produces CO2. (This occurs continuously, day and night).
Define Net Change:Net CO2 Uptake=Rate of Photosynthesis−Rate of Respiration
Analyze the scenario:
The sensor shows Net CO2 Change = 0.
Therefore, 0=Rate of Photosynthesis−Rate of RespirationRate of Photosynthesis=Rate of Respiration
Conclusion: The student is incorrect. Photosynthesis has not stopped. Instead, it is occurring at the exact same rate as cellular respiration. The CO2 released by respiration is immediately recycled and used for photosynthesis. This specific light intensity is known as the Compensation Point.
Question:
When a desk lamp is placed at a distance of 10 cm from a Hydrilla setup, the plant produces 40 bubbles/min. When the lamp is moved further away to a distance of 30 cm, the light intensity drops significantly, and the plant produces only 10 bubbles/min. Calculate the percentage decrease in the rate of photosynthesis.
Step-by-Step Solution:
Identify the Given Rates:
Initial Rate (R1) = 40 bubbles/min
Final Rate (R2) = 10 bubbles/min
Calculate the Absolute Decrease:Decrease=R1−R2=40−10=30 bubbles/min
Calculate the Percentage Decrease:Percentage Decrease=(Initial RateDecrease)×100Percentage Decrease=(4030)×100=0.75×100=75%
Answer: The rate of photosynthesis decreased by 75% when the light source was moved further away.
Question:
A tomato plant is grown in a greenhouse. At 20∘C and 0.03%CO2, increasing the light intensity from 1000 lux to 3000 lux increases the photosynthetic rate from 15 to 45 units. However, increasing light intensity from 3000 lux to 5000 lux keeps the rate stagnant at 45 units.
If the greenhouse temperature is then raised to 30∘C while keeping the light at 5000 lux, the rate jumps to 60 units.
Identify the limiting factor between 3000-5000 lux before the temperature was raised.
Identifying the Limiting Factor:
Increase Light
If Rate Increases ➔ Light is the Limiting Factor.
If Rate Does Not Increase ➔ Move to Step 2.
Increase Temp or CO₂
If Rate Increases ➔ Temp or CO₂ was the Limiting Factor.
If Rate Remains Stagnant ➔ Another Unknown Factor is Limiting.
Step-by-Step Solution:
Analyze Blackman's Law of Limiting Factors: If a process depends on multiple factors, its rate is limited by the pace of the slowest factor.
Examine Interval 1 (1000 to 3000 lux at 20∘C): The rate increases proportionally as light increases. Light is the limiting factor here.
Examine Interval 2 (3000 to 5000 lux at 20∘C): Light increases, but the rate is stagnant (45 units). The curve has plateaued. This means light is no longer limiting; there is enough light, but some other factor is holding the rate back.
Examine the change (Temperature increase to 30∘C at 5000 lux): Raising the temperature caused the rate to increase to 60 units. This proves that at 20∘C, the temperature was the bottleneck preventing the rate from going higher despite abundant light.
Answer: The limiting factor between 3000 and 5000 lux (at 20∘C) was Temperature.